Figure 2

Expression of taste bud cell lineage markers in the organoid.

(a) Sectioned CV tissue or organoid were stained with UEA1 (red), E-cadherin (green) and T1R3 (red) or E-cadherin (green) and gustducin (red), respectively. Cell nucleus (blue) was stained by Hoechst 33342. Scale bars = 50 μm. (b) Transmission electron microscopy images shows the middle of the organoid with low resolution and high resolutions of taste cell (white rectangle). Asterisks indicate the lumen. Scale bars = 10 μm. (c) Sectioned CV tissue (low magnification and high magnification of white rectangle), 3D whole mount organoid, or sectioned organoid (low magnification and high magnification of white rectangle) were stained with SOX9 (red). Nuclei (blue) was stained with Hoechst 33342. Scale bars = 50 μm.