Figure 3

Lgr5⁺ or CD44⁺ cell-induced taste bud organoids.

(a) 3D images CV region of tongue freshly isolated from Lgr5-GFP/membrane-tdTomato mouse with low magnification and high magnification of white rectangle indicated in top panel. Scale bars = 50 μm. (b) Sectioned Lgr5-GFP mouse CV tissue was immunostained for GFP (green), CD44 (red) and nuclei (Hoechst 33342: blue). Scale bars = 50 μm. (c) Representative histograms of Lgr5-GFP stem cell sorting from isolated CV tissue and expression of Lgr5 or CD44 mRNA were determined. Images of taste bud organoid growth from sorted Lgr5-GFP stem cell, at indicated days after cell plating. (d) Sectioned organoid was stained with CD44 (green), SOX9 (red) and nuclei (blue). Scale bars = 50 μm. (e) Representative histograms of CD44⁺ cell sorting from taste bud organoids and expression of Lgr5 or CD44 mRNA were determined. Images of taste bud organoid growth from sorted CD44⁺ cell. (f) After 12 days culture of Lgr5-GFP cell or CD44⁺ cell-derived taste bud organoid, stem/progenitor cell or taste bud lineage markers mRNA was determined by RT-PCR.