Figure 4
From: Site Specific Genetic Incorporation of Azidophenylalanine in Schizosaccharomyces pombe
NanoLC-ESI-MS/MS Analysis of Glutathione-S-transferase containing the incorporated amino acids.
(A) The spectra of tryptic peptide MSPILG*YWK incorporating into tyrosine at glycine 6th (denoted G*) is shown with its expected fragment ion mass y4 (in red square). Parent error:-0.50 ppm. (B) The spectra of tryptic peptide MSPILG*YWK incorporating into unnatural amino acid—amino-phenylalanine at glycine 6th (denoted G*) is shown with its expected fragment ion mass y4 (in red square). Parent error:-3.5 ppm.
