Figure 5
From: Site Specific Genetic Incorporation of Azidophenylalanine in Schizosaccharomyces pombe
Cross-linking induction with GST (F52AzF)-6HIS.
(A) S. pombe cells expressing E. coli TyrRS (MBY 8570) or E. coli AzFRS (MBY 8487), both were transformed with E. coli tRNATyr and GST with a chain terminating UAG stop codon at amino acid position 52 (a site known to be involved in GST dimerization). GST was purified following non-sense UAG suppression. Purified GST from all four samples were analyzed by GST antibody. Please refer to Supplemental Table S1 for the strain number. (B) The purified GST was exposed to UV light at 254 nm for 5 min and western blot with GST antibodies.
