Table 1 Summary of in situ click chemistry experiments and dissociation constants.

From: A new application of click chemistry in situ: development of fluorescent probe for specific G-quadruplex topology

Compound

In situ hita (HRAS)

In situ hita (c-kit2)

(μM)

(μM)

9

Yes

Yes

6.0

1.8

10

Yes

Yes

5.6

2.0

11

No

No

8.0

4.8

12

Yes

Yes

7.1

2.7

13

Yes

Yes

12.9

4.3

14

No

No

25.9

3.1

15

No

Yes

−b

6.1

1

−

−

37.9

13.9

  1. aIn situ hit represents the compound whose formation was accelerated by addition of the G-quadruplex template.
  2. bNo significant binding was found for addition of up to 40 μM ligand.
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