Figure 3
From: The role, mechanism and potentially novel biomarker of microRNA-17-92 cluster in macrosomia

miR-17-92 cluster miRNAs inhibited expression of SMAD4 and RB1 in HTR8/SVneo cells.
(A) Detection of SMAD4, SMAD3, RB1 and EP300 mRNA expression in miR-17-92 mimics or miR-17-92 inhibitors-transfected HTR8/SVneo cells by qRT-PCR and normalized to GAPDH. (B) Detection of SMAD4, SMAD3, RB1 and EP300 protein expression by Western-blot. Data were normalized to the level of GAPDH. *P < 0.05; **P < 0.01; compared with the control.