Figure 5

miR-17-92 cluster miRNAs enhanced the proliferation of HTR8/SVneo cells.

The effect of miR-17-92 cluster miRNAs on cell proliferation was detected using CCK-8 and EdU after transfection with miR-17-92 mimics or inhibitors. (A) Phase contrast microscopy of HTR8/SVneo cells treated with miR-17-92 mimics or inhibitors for 24h. (B) The effect of miR-17-92 cluster miRNAs on cell proliferation was detected at 24h and 48h using CCK-8. (C) Cell proliferation was determined by the EdU assay. Forty-eight hours after transfection, HTR8/SVneo cells were stained with EdU and DAPI. The percentage of EdU-positive HTR8/SVneo cells was quantified. Data are expressed as the mean ± SEM (n = 5). *P < 0.05; **P < 0.01; compared with the control.