Figure 6
From: A new live-cell reporter strategy to simultaneously monitor mitochondrial biogenesis and morphology
Quantitative analysis of mitochondrial dynamics.
Untreated (Ctr) and AICAR-treated (0.5 mM, 6 days) HeLaNRF1/c4 cells were imaged as described in Fig. 4. CCCP (5 μM, 30–60 min) was added to induce mitochondrial fragmentation. Data were extracted from analysis of single cells and are shown as and mean ± SEM. (A) Number of mitochondria relative to biomass (Ncell/Vm,cell ratio). The number of cells analysed for each treatment is shown. (B) The relative amount (percentage) of the mitochondrial biomass (volume) found in the three different size-populations. (C) The relative number (percentage) of the mitochondria found in the three different shape-populations. *p < 0.05 compared with Ctr, #p < 0.05 compared with Ctr+CCCP.
