Figure 5
From: Multimodal targeted high relaxivity thermosensitive liposome for in vivo imaging
Targeting and in vivo imaging of tumors with biotin-NLP-RPE.
Gli36 human glioma cells or same cells expressing biotin on their surfaces (through BAP-TM) were implanted subcutaneously in nude mice (n = 5) at 2 different sites. Two-weeks later, mice were i.v. injected with either biotin-NLP-RPE or a complex consisting of biotin-NLP-RPE and streptavidin-Alexa750 (Alexa750-SA-NLP-RPE) and imaged 4 hrs later with FMT Alexa750. (a,b) A representative mouse from each group is shown in (a) and quantitation of fluorescent accumulation in each tumor in (b). (c,d) The same mice were also imaged with T1w MRI at 4 and 24 hrs post-injection (c). T1w intensities at 24 hrs were normalized to 4 hrs for each tumor (d). *p < 0.001 as calculated by student’s t-test.
