Figure 4
From: Normothermic Mouse Functional MRI of Acute Focal Thermostimulation for Probing Nociception
BOLD activation maps.
Left panel: Anatomical mouse brain atlas in Waxholm space (WHS) superimposed on a high-resolution reference model. Structural elements of the pain matrix are displayed in white: the paw region of the primary somatosensory cortex (S1) composed of areas representing hindpaw (H) and forepaw (F), the barrel field area of S1 (S1BF), cingulate cortex (Cg), secondary somatosensory cortex (S2) and insular cortex (Ins). Hippocampal subfields CA1 and CA3 and caudate /putamen (CPu) are colored respectively (see color legend). Partial overlaps of anatomical regions result from maximum intensity projection during dimensionality reduction in z-direction (see Online Methods). Mid panel: Z-statistic parametric map from a mixed effects analysis of 6 mice in response to heat stimulation of the plantar hindpaw (FWE, p < 0.05) projected onto a structural reference scan. Significant clusters were found i.a. in structures of the pain matrix including the cingulate cortex (subdivided in anterior cingulate cortex (ACC) and retrosplenial cortex (RSC)), S1, S1BF, S2, thalamus (Th) including the habenula (Hbn) and the insular cortex (Ins). Arrows are used to indicate significant clusters in hippocampus (black) and CPu (white). Ipsi- and contralateral sides are labeled above. Right panel: Relative signal intensity change over time for significant voxels within defined anatomical brain regions (mean ± 2 s.e.m.). ROIs were defined based on significant clusters, which were masked by selected anatomical structures (see Online Methods).
