Figure 2

Analysis of the spontaneous activity.

(A) Representative raster plots of spontaneous activity and average fluorescence signal, comparing the behavior of the clustered neuronal networks in control conditions (top) and under perturbation with M-Aβ complex (bottom). The left panels show the first 10 min of recording before perturbation, while the right panels show the last 10 min of recording upon perturbation. For the control case, the network activity and the structure of the different firing sequences (outlined boxes) are preserved, while for the M-Aβ case both traits degrade, with a rupture of the biggest sequence in sub-sequences and the silencing of clusters (dotted horizontal lines). (B) Variation of the mean clusters’ firing rate upon perturbation and comparing the action of M, Aβ and M-Aβ. The left panel shows the values before and after application and corrected by the control measurement; the right panel shows the corresponding ratios. (C) Complementary analysis considering the sequences’ occurrence rate . Each dataset is an average over 15 experimental realizations and is represented as mean +/− standard error of the mean. (*p < 0.05, **p < 0.005, Student’s t-test.) The red horizontal line is a guide to the eye for the control condition.