Figure 1 | Scientific Reports

Figure 1

From: Human Granulocyte Macrophage Colony-Stimulating Factor Enhances Antibiotic Susceptibility of Pseudomonas aeruginosa Persister Cells

Figure 1

GM-CSF sensitized the persister cells of P. aeruginosa PAO1 to antibiotics.

The wild-type PAO1 obtained from two different sources were tested including one (a) from Prof. Thomas K. Wood at Pennsylvania State University and another (b) from Prof. Matthew Parsek at University of Washington. The persister cells were isolated from exponential phase cultures by killing the normal cells with 200 μg/mL ciprofloxacin for 3.5 h and then treated with 0.17 pM GM-CSF alone for 1 h, followed by additional treatment with GM-CSF plus an antibiotic as indicated for 3.5 h (all tested at 200 μg/mL). The samples without GM-CSF or antibiotic were used as controls. The amount of BSA (0.1%) was adjusted to be the same for all samples. Following the treatment, the viability of persister cells was determined by counting CFU. Cip: ciprofloxacin. Tob: tobramycin. Tet: tetracycline. Gen: gentamicin. The samples were tested in triplicate (n = 3). Error bars represent SD; **p < 0.01, ***p < 0.001, ****p < 0.0001, one-way ANOVA followed by Tukey test.

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