Figure 2

BicA and SbtA expression via His tag (a) and the uptake of HCO3− in E. coli AFP111 (b). Overexpressed proteins were detected by western-blots. Lane 1: Tang1505 (pTrc99A), lane 2: Tang1506 (sbtA), lane 3: Tang1507 (bicA), lane 4: Tang1508 (sbtA and bicA). M corresponds to the molecular weight marker lanes. Symbols for E. coli strains: Tang1505 (pTrc99A) (open triangle, ), Tang1506 (sbtA) (black triangle,
), Tang1507 (bicA) (open circle, ○), Tang1508 (sbtA and bicA) (black circle,
).