Figure 4
Time courses of dry cell weight (a), residual sugar concentration (b) and the production of succinate (c), relative expression levels of sbtA, bicA, ppc and pck (d) and the specific activities of the PCK and PPC (e) under the collaborative expression of multiple HCO3− transporters and CO2 fixation genes in fed-batch fermentation. Symbols for E. coli strains: Tang1509 (pTrc99A and pACYC184) (open triangle, 
), Tang1510 (sbtA, bicA and ppc) (black triangle, 
), Tang1511 (sbtA, bicA and pck) (open circle, ○), Tang1512 (sbtA, ppc and pck) (black circle, 
), Tang1513 (bicA, ppc and pck) (open square, 
), Tang1514 (sbtA, bicA, ppc and pck) (black square, 
). Different letters (e.g., (a–c)) were assigned to significantly different groups and for the results between two groups, a combination of the two corresponding letters was used (e.g., a,b and b,c).
