Table 1 Strains and plasmids used in this study.
Strains | Relevant characteristics | Sources or reference |
|---|---|---|
AFP111 | F + λ- rpoS396(Am) rph-1 ΔpflAB::Cam ldhA::Kan ptsG | 20 |
Synechocystis PCC6803 | Providing sbtA, bicA and ppc gene | 21 |
Bacillus thuringiensis BMB171 | A crystalliferous B. thuringiensis mutant, providing pck gene | 22 |
DH5α | F-φ80 lacZΔM15Δ(lacZYA-argF)U169 end A1 recA1 hsdR17(rk-,mk-) sup E44 λ-thi-1 gyrA96 relA1 phoA | TransGen Biotech |
Tang1501 | AFP111/pACYC184 | This study |
Tang1502 | AFP111/pTrc-ppc | This study |
Tang1503 | AFP111/pACYC-pck | This study |
Tang1504 | AFP111/pTrc-ppc + pACYC-pck | This study |
Tang1505 | AFP111/pTrc99A | This study |
Tang1506 | AFP111/pTrc-sbtA | This study |
Tang1507 | AFP111/pTrc-bicA | This study |
Tang1508 | AFP111/pTrc-bicA-sbtA | This study |
Tang1509 | AFP111/pTrc99A + pACYC184 | This study |
Tang1510 | AFP111/pTrc-ppc-bicA-sbtA | This study |
Tang1511 | AFP111/pTrc-bicA-sbtA + pACYC-pck | This study |
Tang1512 | AFP111/pTrc-ppc-sbtA + pACYC-pck | This study |
Tang1513 | AFP111/pTrc-ppc-bicA + pACYC-pck | This study |
Tang1514 | AFP111/pTrc-ppc-bicA-sbtA + pACYC-pck | This study |
Tang1515 | AFP111/pTrc-ppc-sbtA | This study |
Tang1516 | AFP111/pTrc-ppc-bicA | This study |
Tang1517 | AFP111/pTrc-sbtA + pACYC-pck | This study |
Tang1518 | AFP111/pTrc-bicA + pACYC-pck | This study |
Plasmids | ||
pTrc99A | ApR, pBR322 ori, trc promoter, lacIq | Invitrogen |
pTrc-sbtA | pTrc99A with sbtA gene | This study |
pTrc-bicA | pTrc99A with bicA gene | This study |
pTrc-bicA-sbtA | pTrc99A with sbtA and bicA gene | This study |
pTrc-ppc | pTrc99A with ppc gene | This study |
pTrc-ppc-sbtA | pTrc99A with ppc and sbtA gene | This study |
pTrc-ppc-bicA | pTrc99A with ppc and bicA gene | This study |
pTrc-ppc-bicA-sbtA | pTrc99A with ppc, sbtA and bicA gene | This study |
pACYC184 | catR, tetR, p15A ori | NEB |
pACYC-pck | pACYC184 with trc promoter and pck gene | This study |
