Table 2 Primers used in this studya.
Primer sets | Relevant characteristics | Sources |
|---|---|---|
SbtA-SacI-H | GACCGAGCTCATGGATTTTTTGTCCAATTTCTTGACGGACTTCGTGGG | This study |
SbtA-B-His | GAAGGATCCTTAGTGATGGTGATGGTGATGACCTGCACCAAGGGTCTGGGC | This study |
BicA-EcoRI-H: | GACGGAATTCATGCAAATAACTAACAAAATTCATTTTAGGAACCTGCAGGGGGA | This study |
BicA-B-His | GAAAGGATCCTTAATGGTGATGGTGATGGTGGTATGTGGTCTGGACGGAAG | This study |
P-trc-XbaI | GCCTCTAGATGACAATTAATCATCCGGCTCGTATAATGTGTGG | This study |
SbtA-SalI-His | GACGTCGACTTAGTGATGGTGATGGTGATGACCTGCACCAAGGG | This study |
ppc-EcoRI | CCGGAATTCGATATGAACTTGGCAGTTCCTGCATTCGG | This study |
ppc-BamHI | ACCGGATCCTCAACCAGTATTACGCATTCCGGCCGC | This study |
BicA-salI | GGTCGACTTAATGGTGATGGTGATGGTGGTATGTGGTCTGG | This study |
BamHI-SD-BicA | GACGGGATCCAGGAGGATGCAAATAACTAACAAAATTCATTTTAGGAACC | This study |
BicA-XbaI | GATTTCTAGATTAATGGTGATGGTGATGGTGGTATGTGGTCTGGACGGAAG | This study |
pck-SacI | CGAGCTCATGCGAAATGAAGGGAAATT | This study |
pck-HindIII | CCCAAGCTTTTAAGCGATTGGACCGCCTA | This study |
trc-pck-DrdI | CGCGACATCGAAGTCGCAGGTCGTAAATCACTGC | This study |
trc-pck-BclI | CGCTGATCATTAAGCGATTGGACCGCCTAAG | This study |
SbtA-F(RT) | GCATGGCAATTCGGAACTCCAAC | This study |
SbtA-R(RT) | CAGCCATTGTAGAGCCACTGACTG | This study |
BicA-F(RT) | CAGGGCATCGGCAATGTAATGTC | This study |
BicA-R(RT) | GAATGGTAGCCGCCAATTTAGCTG | This study |
PCK-F(RT) | TTTGCAGGCGCTGACCGCAATTAC | This study |
PCK-R(RT) | CAGCTGGATCAGCTTTGAAGTTCG | This study |
PPC-F(RT) | AACCATTGCCAGTGGGCATTGAC | This study |
PPC-R(RT) | CCGGATGGTGTGACGGACAATTTC | This study |
16S rRNA-F | GCTAATACCGCATAACGTCGCAAG | This study |
16S rRNA-R | GGACCGTGTCTCAGTTCCAGTGTG | This study |
