Figure 3
Morphometric indicators were generally dependent on PAH concentrations.
Edema area (A) and sinus venosus–yolk mass (SV–YM) gap (B) were quantitative measures related to the severity of edema, atrioventricular (AV) angle (C) was a measure of heart morphology (chamber looping) and fractional shortening (D) was a functional measure of contractility based on chamber diameters. All measures were made in digital video frames (see Methods) and plotted with lines representing non-linear (A,D) or linear regression (B,C) models. As described in the text, these regressions were used for graphical representation only and not statistical analyses (e.g., Figs 4 and 5, Supplemental Materials: Table S1). ∑PAH40 concentrations represent actual measured values of HEWAF dilutions including both particulate whole oil and dissolved PAHs. Data are mean ± SEM.
