Figure 6
Expression of molecular cardiotoxicity indicators across developmental time in Control and AW-Source HEWAF exposed mahi mahi larvae.
(A–H,J) Quantitative PCR (qPCR) determined transcript abundance (i.e., Ct values) of nine cardiotoxicity indicator genes (A, amhc; B, cmlc2; C, fhl2; D, gata4; E, nkx2.5; F, nppa; G, nppb; H, tbx5; J, vmhc) and hsp70 (K) as a stress indicator across developmental time (48–58 hpf) in control and 0.10% AW-Source HEWAF exposed larvae. Gene-specific p values are from two-way ANOVA (Supplemental Materials: Table S2) and letter groupings indicate time points with statistically different expression levels (Tukey-Kramer HSD post-hoc, α = 0.05). Presented Ct values were corrected to geometric average of actc1b and rps25 following Reference Residual Normalization58. (I) Legend depicting orientation of Y-axes and conversion of Ct to fold-change. Open symbols, control; Filled symbol, exposed. Ct, threshold cycle. Y-axes range 5 cycles or 32-fold. Data are mean ± SEM.
