Figure 7
In situ hybridization showing endogenous localization of qPCR amplicon DIG-riboprobes in larval mahi mahi.
Ventricular myosin heavy chain (vmhc) and cardiac myosin light chain 2 (cmlc2) in situs show ventricle- and heart-specific localization, respectively. (A,C) Control larvae exposed to 0.8 μg/L ΣPAH (n = 12). (A′,C′) Larvae exposed to 6.2 μg/L ΣPAH Slick A HEWAF (n = 13). (B,D) Histograms depicting area-corrected mean pixel intensity of ventricle- and cardiac-specific expression of vmhc (B) and cmlc2 (D), respectively. Asterisks denote significant difference between control and HEWAF exposed (Student’s t-test, p < 0.05).
