Figure 4
From: Genetic Factors for Enhancement of Nicotine Levels in Cultivated Tobacco
Expression patterns of nicotine synthetic genes measured by qRT-PCR.
(a) The average expression level of T0 plants is shown. Two T0 plants from each construct that affects the nicotine level most were analyzed (AOE-3, AOE-6 for NtMYC2a and BOE-16, BOE-17 for NtMYC2b). (b) The average expression levels of nicotine synthetic genes in T1 plants are shown. Two transgene positive plants of each T1 line and thus total four plants for each construct were analyzed. The expression level in the vector control was set to 1 and expression of nicotine synthetic genes in T0 and T1 transgenic plants is shown relative to this level, the error bars indicate standard errors. AOE: NtMYC2a overexpression; BOE: NtMYC2b overexpression.
