Figure 4

(A) Viabilities of 4T1 cells were estimated by the CCK-8 proliferation method versus incubation concentrations (3.6, 6.3, 12.5, 25, 50, 100 and 200 μg/mL) of the solutions of MoS₂-PEG nanoflakes. Cells were incubated with the solution of MoS₂-PEG nanoflakes at 37 °C for 24 and 48 h and treated with PBS were used as control. Data presented as mean ± standard deviation (n = 5). (B) Hemolytic percent of RBCs treated with MoS₂-PEG nanoflakes with different concentrations, using deionized water (+) and PBS (−) as positive and negative controls, respectively. Inset: Photographs for direct observation of hemolysis. (C) The cell viability assay of 4T1 cells after exposed to different concentrations of MoS₂-PEG nanoflakes with or without the irradiation of NIR 808-nm laser (1 W/cm², 10 min). (D) Cell viability assay of 4T1 cells after treated with or without MoS₂-PEG nanoflakes (60 μg/mL) under the irradiation of different NIR 808-nm laser power density. Data presented as mean ± standard deviation (n = 5).