Figure 5

(A) Optical microscopy images of trypan blue stained 4T1 cells after incubation with different conditions as denoted in each individual image. Only destruction or dead cells can be stained to be blue. (B) Confocal laser scanning microscopy (CLSM) images of acridine orange (AO) stained 4T1 cells after incubation with different conditions as denoted in each individual picture. (C) CLSM images of Alexa Fluor®568 conjugated phalloidin (red) stained cells cytoskeleton after incubation with different conditions as denoted in each individual picture. (D) CLSM images of DAPI (blue) stained cell nuclei for clear observation after cells incubation with different conditions as denoted in each individual picture. (E) Representative H&E stained histological images of corresponding tumor sections from the tumor with different treatments. (a) Blank control (neither MoS₂-PEG nanoflakes nor the NIR-laser treatment). (b) Only MoS₂-PEG nanoflakes without NIR 808-nm laser was used treatment with 4T1 cells. (c) Only NIR 808-nm laser without MoS₂-PEG nanoflakes was used treatment with 4T1 cells. (d) MoS₂-PEG nanoflakes treated cells are irradiated by NIR 808-nm laser.