Figure 3
From: Tuneable endogenous mammalian target complementation via multiplexed plasmid-based recombineering
Highly efficient wild-type down-regulation and concomitant mutant complementation for BRAF, HRAS and SHP2 proteins by TEMTAC.
(a) Expression and phenotype analysis of BRAF V600E and WT in HEK293 cells is shown. Left panel: Western blot results using specific antibodies as indicated. Right panel: MEKp quantification after intensity analysis using ImageJ. (b) Expression and phenotype analysis of HRAS G12V and WT in HEK293 cells. Left panel: Western blot results using antibodies as indicated. Right panel: MEKp quantification after intensity analysis using ImageJ. (c) Expression and phenotype analysis of SHP2 C459G, D61G and WT in GH-HEK293 cells (HEK293 cells stably expressing the growth hormone receptor). Left panel: Western blot results using antibodies as indicated. Right panel: ERKp quantification after intensity analysis using ImageJ. The dashed line indicates that the blot has been cropped. The full blot is provided in Supplementary Figure S11.
