Figure 1
Distributions of WMS sequencing reads and microbial communities within bile samples.
(a) Ratios of WMS sequencing reads after removing host background (hollow black-edged bars) and ratios of reads aligned to bacterial database (inner bars). All ratios were calculated by dividing by the total clean read number after quality control. Sample IDs are labelled on the x-axis and ranked by their clustering relationships. Bacteria-aligned read ratios of samples from groups A, B and C are indicated by light blue, dark blue and green bars, respectively. (b) Hierarchical clustering of samples based on microbial community, distribution of bacterial origin and heatmap of species abundances. Individuals are denoted by coloured blocks, as in (a). Bacterial origins were classified as oral (referring to the oral cavity/respiratory tract), intestinal, both oral and intestinal and other (environmental or unknown). For bacteria with ≥0.1% abundance in at least three individuals, the distribution of their origin in each individual is reflected by the histogram above the heatmap. The heatmap colour scale quantifies the log10 relative abundance of species, from grey (none or low abundance) to dark red (high abundance).
