Figure 2
From: Acetaminophen cytotoxicity is ameliorated in a human liver organotypic co-culture model
Hepatic functionality of C3As in mono- and co-culture.
Determination of (A) albumin synthesis and (B) CYP3A4 activity in C3A monocultures and C3A:HUVEC co-cultures. Data is expressed as the mean ± SEM (n = 3), *p < 0.05, **p < 0.01. (C) Phase contrast and fluorescence images of C3A and HUVEC monocultures or C3A:HUVEC co-cultures stained with antibodies specific for F-actin (red), CYP3A4 (green) and counterstained with DAPI (blue). Arrows indicate examples of F-actin bands surrounding morphological bile canalicular structures confirming the presence of differentiated cellular polarity seen during albumin production. Scale bars = 100 μm.
