Figure 3
From: Acetaminophen cytotoxicity is ameliorated in a human liver organotypic co-culture model
Mitochondrial dysfunction and cell viability in C3A and HUVEC mono- and co-cultures following 24 h APAP exposure.
Assessment of (A) cell viability by measurement of total intracellular ATP content in C3A and HUVEC monocultures or C3A:HUVEC co-cultures and (B) mitochondrial dysfunction by measurement of ATP generated following 90 minutes incubation with D-(+)-galactose, following exposure to 0 mM (untreated control), 5 mM, 10 mM, or 20 mM APAP for 24 hr. Data is expressed as the percentage mean ± SEM (n = 3), *P < 0.05, **p < 0.01. Phase contrast and fluorescence images of (C) C3A monocultures; (D) HUVEC mono cultures; or (E) C3A:HUVEC co-cultures subjected to live (bright green)/dead (bright red) viability staining, either following treatment with 0 mM (untreated control) or 10 mM APAP for 24 hr. Red and green stained images were merged using ImageJ 1.46r. Magnification ×10; Scale bars = 100 μm.
