Figure 4
From: Acetaminophen cytotoxicity is ameliorated in a human liver organotypic co-culture model
Pyruvate/lactate ratios in C3A and HUVEC mono- and co-cultures treated with APAP followed by NAC.
Determination of (A) pyruvate/lactate ratios or individual values for (B) lactate and (C) pyruvate in C3A (black bars) and HUVEC (white bars) mono-cultures or C3A/HUVEC co-cultures (patterned bars) treated with 10 mM-APAP for 24 hr followed by culture in EGM-2 for 24 hr (10 mM-APAP), before cells were treated with 10 mM-APAP for 24 hr followed by 5 mM-NAC for a final 24 hr (5 mM-NAC), or cultured in EGM-2 for both periods (control). Pyruvate/lactate ratios and values for individual metabolites are displayed as the mean ± SEM (n = 3); *p < 0.05, **p < 0.01, ***p < 0.001.
