Figure 2
From: A Novel Inhibitor Prevents the Peripheral Neuroparalysis of Botulinum Neurotoxins
Immunocytochemical evaluation of EGA inhibition against different serotypes of BoNT in CGNs.
(a–c) CGNs were treated with EGA 12.5 μM or vehicle (DMSO) at 37 °C for 30 min. Thereafter, the indicated amount of BoNT was added for 12 hrs. Samples were fixed and stained with an antibody specific for BoNT/A-cleaved SNAP25 (a) or intact VAMP2, (b,c). BoNT/A-cleaved SNAP25 was detected with an Alexa Fluor 555 goat anti-rabbit, while VAMP2 with an Alexa Fluor 488 goat anti-mouse. Images shown are representative of three independent experiments. Scale bar, 10 μm.
