Figure 3
From: A Novel Inhibitor Prevents the Peripheral Neuroparalysis of Botulinum Neurotoxins
EGA interferes with the BoNT/A,/B and/D toxicity in CGNs.
(a,c,e) CGNs were preincubated for 30 min with the indicated concentrations of EGA at 37 °C. Where indicated, BoNTs were added at the reported concentrations for 12 hrs. Then cells were lysed and the SNARE content was estimated with the indicated antibodies: (a) SMI81 recognizes both the full length and the cleaved form of SNAP25; BoNT/A-cleaved recognizes only BoNT/A-truncated SNAP25; (c,e) VAMP2 (69.1) recognizes the intact form of VAMP2 and, BoNT/B-cleaved recognizes only BoNT/B-cleaved VAMP2. In all experiments an antibody against the Na+/K+ ATPase antibody was used as loading control. Blots are representative of a typical experiment. (b,d,f) Densitometry analysis of western blots obtained in (a,c) and (e) respectively. All data are presented as mean values and error bars indicated the standard deviation obtained from at least three independent experiments.
