Figure 6
From: A Novel Inhibitor Prevents the Peripheral Neuroparalysis of Botulinum Neurotoxins
EGA provides protection against different serotypes of BoNTs in the phrenic nerve-hemidiaphragm twitch model, delays death induced by BoNT/A and strongly protect against death induced by BoNT/B and BoNT/D.
(a–c) For each experiment, 12.5 μM EGA or vehicle (DMSO) was added to the nerve-muscle preparations in the bath at 37 °C; after 30 min, 10 pM BoNT/A (a) or 10 pM BoNT/B (b) or 100 pM BoNT/D (c) was added (time = 0). Muscle twitch was induced by nerve stimulation and monitored until paralysis. A representative experiment is reported for each toxin, showing the progressive twitch decrease in the presence of vehicle (black trace) or EGA (red trace). (d–f) Each experiment performed for (a–c) was expressed as the time required to decrease the twitch to 50% of the initial value (paralysis half time) in vehicle (black points) or EGA treated muscles (red points). (g–i) Adult CD1 mice preconditioned with EGA 12.5 mg/Kg (n = 20) or vehicle alone (n = 20) were i.p. injected with 2 × MLD50 of BoNT/A (g) or BoNT/B (h) or BoNT/D (i). The animals were monitored every 4 hrs for 96 hrs. The survival curves were compared and found to be significantly different (p < 0.0001).
