Figure 5
From: The isolation and characterization of CTC subsets related to breast cancer dormancy
Single-cell DEPArray™ isolation of uPAR/int β1 CTC subsets from breast cancer patients.
Multiparametric flow cytometry (Six fluorescence channels, ARIA IID system, BD Biosciences™) was applied to select EpCAM-negative/CD45−/CD44+/CD24− CTC followed by DEPArray single-cell isolation to select a combinatorial expression of uPAR (FITC), int β1 (ApC) and human epidermal growth factor receptor-2 (HER-2) (PE). DEPArray™ (Silicon Biosystems, Inc.) analyses were subsequently performed by Cell BrowserTM software. Representative single CTCs captured and isolated by DEPArray™ are shown. DAPI (ThermoFisher Scientific; cat # D1306) = nuclear staining blue. BF = Brightfield.
