Figure 7
From: The isolation and characterization of CTC subsets related to breast cancer dormancy
3D invasion assays of in vitro 3D CTC tumorspheres.
(a) Experimental strategy with steps on 3D cell culture 96-well BME cell invasion assays; (b) Four CTC tumorspheres with breast cancer no brain metastasis were trypsinized and dissociated as single CTC units or pairlet cells. Control consisted of non-invasive MCF7 and invasive 231Br breast cancer cells. Images were captured at endpoint under 40× magnification using phase contrast microscopy (Zeiss, Inc.). Scale bars, 25 μM. Representative images of three independent experiments are shown.
