Figure 8

Embryonic stem cell gene expression profiling.

PBMCs subpopulation of breast cancer patient with and without brain metastasis were sorted by FACS. uPAR^+/− and int β1^+/− population were collected respectively (containing EpCAM-negative/CD45⁻/CD44⁺/CD24⁻expression markers). RNA were extracted, amplified and real-time PCR analysis were performed using RT²-PCR embryonic stem cell array profiler (Qiagen). The change in mRNA expression (>3 fold) is shown comparing uPAR⁺/int β1⁺ population to uPAR⁻/int β1⁻ population in CTCs isolated from patients clinically diagnosed with BCBM (a) or without BCBM (b).