Figure 4
Potentiation of mTOR signalling by follistatin is impaired in denervated muscles.
The effect of rAAV:Fst administration at the time of denervation on abundance of (A) phosphorylated Akt (p-Akt), (B) phosphorylated mTOR (p-mTOR), (C) phosphorylated S6RP (p-S6RP), (D) total Akt, (E) total mTOR and (F) total S6RP protein. Muscles were analysed 2 weeks after vector administration and surgery. Total SMAD4 protein levels were determined as a loading control and are displayed in Figures 4G–4I. Representative blots presented in Figures 4J–4L. ***main effect for denervation on protein levels (p < 0.001); #and ###main effect of rAAV:Fst treatment on protein levels (p < 0.05 or p < 0.001). In (B), (C), (D), (E,F), post-hoc tests revealed a difference in the sham but not in the denervated muscles (p < 0.05).
