Figure 3 | Scientific Reports

Figure 3

From: M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product

Figure 3

AEME competition binding curves obtained in CHO cells expressing individual subtypes of muscarinic receptors (data represent the mean of three independent experiments performed in triplicate).

The Ki values for AEME (μM) were plotted alongside with the competition binding curves. The affinity for rat M2 was greater than rat M4 (p < 0.05), rat M4 was greater than rat M3 (p < 0.05) and rat M1 greater than rat M3 (p < 0.05). There was no difference among rat M4, rat M1 and rat M5. The averages of absolute values of controls are: 2274 ± 187, 888 ± 25, 3698 ± 303, 2831 ± 231 and 4059 ± 415 dpm/25 μg of protein for rat M1, rat M2, rat M3, rat M4 and rat M5, respectively. Data presented as mean ± SEM.

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