Table 1 Saturation binding experiment of [3H]NMS in separate CHO cells expressing one of the five subtypes of mAChRs.

From: M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product

mAChR subtype

Bmax (fmol/mg of protein)

Kd (×10-3 μM)

Rat M1

1087 ± 165

0.085 ± 0.004

Rat M2

1470 ± 053

0.159 ± 0.022

Rat M3

1132 ± 033

0.063 ± 0.008

Rat M4

1835 ± 127

0.038 ± 0.004

Rat M5

1564 ± 027

0.224 ± 0.016

  1. Bmax and Kd values were determined for radioligand competition binding using GraphPad Prism 5 (data represent the mean of three independent experiments performed in triplicate). Data presented as mean ± SEM.
Back to article page