Figure 7
XN represses the association of RANK and TRAF6 and abrogates osteoclastogenesis-related gene expression.
(A,B) XN suppressed the RANKL-induced binding of RANK and TRAF6. The RAW264.7 cells were pretreated with indicated concentration of XN for 4 hours then stimulated with RANKL (30 ng/ml) for another 20 minutes. The cell lysate were harvest and immunoprecipitated with antibody to RANK and then blotted with anti-TRAF6 (A). Or the cell lysates were immunoprecipitated with antibody to TRAF6 and then blotted with anti-RANK (B). (C) XN inhibits the mRNA levels of NFATc1, cathepsin K (CtsK), CTR and TRAP induced by RANKL. Mouse BMMs were incubated with XN (5 μM) and RANKL (30 ng/mg) for indicate day. Total RNA was collected and analyzed by Real time-PCR. Column, means of experiments performed in triplicate; bar, SD. **p < 0.01.
