Figure 3
MiR-181b attenuates scratch, migration and invasion, modulates the epithelial- mesenchymal transition (EMT) of lung cancer cells.
(a) Wound-healing assay in A549/DDP and A549 were determined after transduction with the miR-181b mimics, miR-controls, or miR-181b inhibitors or negative controls. (b) Transwell migration and invasion assays for A549/DDP and A549 were determined after transduction with the miR-181b mimics, miR-controls, or miR-181b inhibitors or negative controls. (c) Western blot detection of E-cadherin, Vimentin, N-cadherin protein expression in A549 and A549/DDP after transduction with the miR-181b inhibitors, negative controls, or miR-181b mimics or miR-controls. GAPDH was used as an internal control. *P < 0.05; **P < 0.01; ***P < 0.001.
