Figure 5 | Scientific Reports

Figure 5

From: Heterologous expression of newly identified galectin-8 from sea urchin embryos produces recombinant protein with lactose binding specificity and anti-adhesive activity

Figure 5

Carbohydrates-binding activity of recombinant Pl-GAL-8.

Hemoagglutination assays on human red blood cells run in duplicates in U-shaped wells. (A) Inhibition of hemo-agglutination was tested by the addition of increasing micromolar (µM) concentrations of: recombinant Pl-GAL-8; BSA, serum bovine albumin; TF, trigger factor; 100 °C, 1 μM of recombinant Pl-GAL-8 denatured at 100 °C for 5 min before testing (negative control). (B) Interaction of mono- or di-sacharide sugars with recombinant Pl-GAL-8 (0.25 µM) at the indicated millimolar concentrations (mM). Captions: lact, lactose; galact, galactose; mann, mannose. Wells showing spread RBC exhibit agglutination activity, wells with small red dots lack agglutination ability. Results shown in (A,B) describe a representative experiment; (C,D) report the mean of 3 replicates from 3 different experiments, +/− SD, expressed in arbitrary units.

Back to article page