Figure 6 | Scientific Reports

Figure 6

From: Heterologous expression of newly identified galectin-8 from sea urchin embryos produces recombinant protein with lactose binding specificity and anti-adhesive activity

Figure 6

Inhibition of HepG2 cells adhesion to culture plates by soluble Pl-GAL-8.

Cells were incubated at 37 °C for 3 h in the presence of increasing micromolar concentrations of soluble recombinant human Galectin-8 (rHs-GAL-8, red line) or recombinant P. lividus-GAL-8 (rPl-GAL-8, blue line). The percentage of cells that remained bound to the wells bottom was determined by measuring the intensity of auto fluorescence developed after several PBS washings and 0.5% glutaraldehyde fixation. Measurements of fluorescence intensities obtained in arbitrary units, are expressed as percentage of adherent cells. Values are presented as the mean of three replicates from a representative experiment and ± SD, standard deviation.

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