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Figure 1

From: Human embryonic stem cell-derived mesenchymal cells preserve kidney function and extend lifespan in NZB/W F1 mouse model of lupus nephritis

Figure 1

hESC-MSC derivation and phenotype.

(A) Schematic outline of the process of differentiation from hESCs to hESC-MSCs. (B) hESC-MSC morphology under bright field imaging (Scale bar 100 μm). (C–H) Flow cytometry analysis of markers positive (C–E) and negative (F–H) for hESC-MSCs. Red histogram represents unstained populations. Average of two independent experiments. (I,J) Isotype control (I) and HLA-G (J) staining of hESC-MSCs (red). Cells were counterstained for DAPI (blue) (Scale bar 50 μm). Staining was performed twice, producing comparable results.

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