Figure 3
From: Epigenomic Reprogramming of Adult Cardiomyocyte-Derived Cardiac Progenitor Cells
Validation of microarray (MA) data by TaqMan low density array (TLDA) and by single-cell TaqMan qPCR assays (qPCR).
(a) PCA plot showing the separation of cardiomyocytes (Ctl, blue) from mouse cardiomyocyte-derived cardiac progenitor cells (mCPCs, red) in their gene expressions as detected by TLDA. (b) Hierarchical clustering of TLDA genes detected in either adult cardiomyocytes (Ctl) or their derived mCPCs with differential expression. (c) Expression level of genes for pluripotency and stemness-related (top panel) and differentiation markers (lower panel) detected by TLDA. For TLDA, the cDNA from each single-cell (6 control adult cardiomyocytes and 8 mCPCs, biological replicates) was run. Normalized probeset intensity exported from Affymetrix Gene Console was used for the calculation of gene expression fold changes detected microarray for 3 single cardiomyocytes and 3 single mCPCs. (d) Expression levels of cardiomyocyte gene Myh6, cardiac progenitor gene c-kit and stem cell gene Sox2 in mCPCs as compared to control cardiomyocytes. N = 5 single cells (mCPCs or adult control cardiomyocytes; technically triplicated for each cell). Note that the preconfigured pluripotency TLDA did not include Myh6 gene.
