Figure 5

Genomic view displaying DNA hyermethylation of Ttn, Myh6 and Myh7 in mCPCs.

(a) Differentially-methylated regions (DMRs) in a portion of the Titin (Ttn) gene detected by CHARM customer array. DMRs were located in both CpG islands and shores (with, or without red marks in the middle panel, respectively). Overlaid onto the CpG density plot is the Ttn gene structure –Ttn is on the minus strand and the gray box represents exon. The upper panel shows the methylation level (1.0 = 100%) of adult cardiomyocytes (Ctl, red) and their derived cardiac progenitor cells (mCPCs, black). The vertical dash lines define the edges of the DMR. The lower panel shows the chromosome locations and differential methylation p values for probes. (b) Genomic view showing differential DNA methylation and differential gene expression between mCPCs and control cardiomyocytes. The gene expression of Myh6 and Myh7 (dash line boxed) genes was down-regulated in mCPCs compared to control cardiomyocytes, whereas their promoter regions were hypermethylated. The top panel shows the Refseq transcript structure; and note that both Myh6 and Myh7 are on the minus strand. Second panel: gene expression fold change (FC) between mCPCs and cardiomyocytes detected by Affymetrix GeneChip gene expression array. The third and fourth panels: transcript and DNA methylation heatmaps. The fifth panel: DMR based on MAT-score on T detected by Roche 3 × 720 k DNA methylation array. The bottom line shows the genome location.