Figure 2
From: Cyclosporine A protects podocytes by regulating WAVE1 phosphorylation
WAVE1 expression increases in podocytes after 24-h PAN treatment.
(A,B) Nephrin and WAVE1 mRNA expression levels were evaluated by quantitative real-time PCR. (C) Nephrin and WAVE1 protein expression levels were measured by Western blotting. (D,E) Protein levels were quantified and normalized to GAPDH expression. (F) Double-immunolabelling of WAVE1 and F-actin in primary cultured podocytes. Scale bar = 20 μm. WAVE1 is labelled in green and F-actin is labelled in red. The data are presented as the mean ± SD. n = 3. *P < 0.05, **P < 0.01.
