Figure 4
Changes in gene regulation and chemokine production following Al(OH)3 treatment.
(A) Heat maps showing the hierarchical clustering of samples along genes in a fixed order for tumor sample in PBS- and Al(OH)3-treated groups. Tumors were harvested from tumor-bearing mice 15 days after the inoculation. Total RNA was extracted and quantitated as described in the methods. The comparison between two groups was carried out using three biological replicates. Shown are transcripts classified as tumor suppression-related and those expression levels significantly different between two groups. (B,C) Balb/c mice were inoculated with105 H22 cells, 5 days later, tumor-bearing mice were randomly divided into two groups. Peritoneal wash fluid and tumor interstitial fluid were collected from mice 3 days after the 1st, 3rd and 6th injection. CCL2, CXCL9 and CCL5 in peritonea (B) and tumor fluid (C) were analyzed by ELISA. Each value represents the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Unmarked comparisons are not statistically significant.
