Figure 5
Neutrophils in tumor.
(A) Mice with established tumors were injected i.p. with Al(OH)3 or PBS. Peripheral blood, spleen, draining LNs and tumor were harvested from tumor-bearing mice on day 3 after the 1st, 3rd and 6th injections. The percentage of neutrophils in myeloid-derived cells or total cells of spleen, draining LNs and tumor were detected by FACS. (B) Neutrophils were purified from tumor-bearing mice in different groups and cocultured with eFluor 450-labeled H22 cells at ratio of 20:1. 12 hour later, tumor cells were gated and their apoptosis was evaluated (right chart). (C) The percentages of tumor cells killed were calculated. Each value represents the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Unmarked comparisons are not statistically significant.
