Figure 1

In vitro network bursts on multielectrode arrays.

(a) A subset of electrodes on a multielectrode array (MEA) imaged under phase contrast conditions to allow the discernment of neuronal somas. The 4 × 4 grid shown here was located in the center of the cell culture. (b) Typical recording of multi-unit activity (MUA) surrounding an electrode (high-pass filtered at 200 Hz). The red trace displays the MUA detection threshold for this channel. (c) Raster plot showing MUA events recorded at each channel for the entire 20 min recording. Grey shaded area is zoomed in below. (d) Same as (c) but zoomed in to show a single network burst (~300 ms). Red circles indicate electrodes identified as burst predictors (i.e., electrodes that spiked <50 ms before a network burst). Green, average population activity. Blue, threshold for the detection of network bursts (see Methods). (e) Raster plot showing electrodes that recorded at least one MUA event before a burst in (c). (f) Burst prediction scores for the data in (e).