Figure 1
Hyperosmotic cryoprotectant solutions increased AQP7, but not AQP3 and AQP9 expression in mouse oocytes.
(A) Immunofluorescence analysis of AQP7 expression in mouse oocytes in the presence of 8% EG, 9.5% DMSO and 0.5 M sucrose, respectively. (B) Summary data of the immunofluorescence analysis in (A). (C) Immunofluorescence analysis of AQP3 expression in mouse oocytes treated as in (A). (D) Summary data of the immunofluorescence analysis. (E) Immunofluorescence analysis of AQP9 expression in mouse oocytes treated as in (A). (F) Summary data of the immunofluorescence analysis. (G) Immunofluorescence analysis of F-actin expression in mouse oocytes treated as in (A). (H) Summary data of the immunofluorescence analysis. Scale bar (A–H), 20 μm. Data in (A–H) are presented as the mean ± SE, n ≥ 6, ** P < 0.01 compared with the corresponding control (Student’s t test). (I) Image of thawed oocytes transfected with scramble siRNA, AQP3 siRNA or AQP7 siRNA after cryopreservation for 48 h with EG as the cryoprotectant. Scale bar, 100 μm. (J) Survival rate of the oocytes after thawing for 2 h. *P < 0.05 and **P < 0.01 compared to the corresponding control (Chi-square test).
