Figure 2 | Scientific Reports

Figure 2

From: Aquaporin7 plays a crucial role in tolerance to hyperosmotic stress and in the survival of oocytes during cryopreservation

Figure 2

Hyperosmosis induced redistribution of AQP7 in the cell membrane.

(A) Immunofluorescence analysis of AQP7 expression in mouse oocytes in the presence of 0.25 M, 0.5 M, 0.75 M and 1 M sucrose, respectively. (B) Summary data of the immunofluorescence analysis (n ≥ 5). (C) Immunofluorescence analysis of AQP7 and F-actin colocalization in mouse oocytes (red: AQP7; green: F-actin). Scale bar (A–C), 20 μm, (D) Co-immunoprecipitation of AQP7 and F-actin. IP, immunoprecipitation. (E) Green fluorescent protein (GFP) intensity in 293FT cells transfected with the GFP-hAQP7 fusion protein expression plasmid or with the GFP vector alone in the presence of 8% EG, 9.5% DMSO and 0.5 M sucrose, respectively. Scale bar, 10 μm. (F) Summary data of the immunofluorescence analysis (calculated for 20 cells for each condition obtained from three independent experiments). Data are presented as the mean ± SE, **P < 0.01 compared to the corresponding control.

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