Figure 3 | Scientific Reports

Figure 3

From: Aquaporin7 plays a crucial role in tolerance to hyperosmotic stress and in the survival of oocytes during cryopreservation

Figure 3

Hyperosmotic cryoprotectant solutions significantly increased CPEB phosphorylation in mouse oocytes.

(A) Immunofluorescence analysis of total CPEB protein level in the presence of 8% EG, 9.5% DMSO and 0.5 M sucrose, respectively. (B) Summary data of the immunofluorescence analysis. (C) Immunofluorescence analysis of phosphorylated CPEB (pCPEB) protein level in mouse oocytes treated as in (A). (D) Summary data of the immunofluorescence analysis (n ≥ 6). (E) Western blotting analysis of phosphorylated CPEB (pCPEB). (F) Summary data of Western blotting analysis (n = 3). Data are presented as the mean ± SE. **P < 0.01 compared to the corresponding control. Scale bar, 20 μm.

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